Virus:Article Title: A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MG1655 CGSC CGSC 6300 E. coli APEC 2248 (APEC) DSMZ DSM 103255 NEB5-alpha competent E. coli New England Biolabs Cat#C2987H vB_EcoM_Goslar Johannes Wittmann (DSMZ) [29] DSM 104658 Chemicals, peptides, and recombinant proteins DAPI (40,6-Diamidino-2Phenylindole,Dihydrochloride) Thermo Fisher Scientific Cat#D1306 FM4-64 Thermo Fisher Scientific Cat#T13320 Ampicillin Thermo Fisher Scientific Cat#BP1760-25 Isopropylthio-b-galactoside (IPTG) Teknova Cat#I3305 Deposited data Mendeley DataSet This study https://doi.org/10.17632/skf8x9kkz8.1 Recombinant DNA pDSW206 plasmid David Weiss [44] N/A Table S1 Software and algorithms DeltaVision SoftWoRx 6.5.2 GE HealthCare incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/7.0.0/SoftWoRx.htm FIJI ver 2.1.0/1.53c ImageJ [45] imagej.net/software/fiji/ GraphPad Prism version 9.2.0 for Mac OS X GraphPad Software, San Diego, California USA. www.graphpad.com MEGA X 10.2.6 MEGA [46,47] www.megasoftware.net Microsoft Excel Microsoft Office www.microsoft.com/en-us/microsoft-365/excel Microsoft Powerpoint Microsoft Office www.microsoft.com/en-us/microsoft-365/ powerpoint Adobe Photoshop Adobe www.adobe.com/products/photoshop.html Adobe Illustrator Adobe www.adobe.com/products/illustrator.html SerialEM ver 3.8b11 [48] bio3d.colorado.edu/SerialEM/ Warp ver 1.09 [49] www.warpem.com/warp/ IMOD Package ver 4.10.28 [50] bio3d.colorado.edu/imod/ MATLAB 2019b MathWorks www.mathworks.com Dynamo ver 1.1514 [51] wiki.dynamo.biozentrum.unibas.ch dynamo2m ver 0.2.2 [52] github.com/alisterburt/dynamo2m RELION ver 3.1.1 [53,54] relion.readthedocs.io/en/release-3.1/ TomoSegMemTV [55] sites.google.com/site/3demimageprocessing/ tomosegmemtv Amira ver 6.7 Thermo Fisher Scientific www.thermofisher.com/us/en/home/electron- microscopy/products/software-em-3d-vis/ amira-software.html ChimeraX ver 1.2.1 [56] www.rbvi.ucsf.edu/chimerax/ ..
Recombinant:Article Title: A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MG1655 CGSC CGSC 6300 E. coli APEC 2248 (APEC) DSMZ DSM 103255 NEB5-alpha competent E. coli New England Biolabs Cat#C2987H vB_EcoM_Goslar Johannes Wittmann (DSMZ) [29] DSM 104658 Chemicals, peptides, and recombinant proteins DAPI (40,6-Diamidino-2Phenylindole,Dihydrochloride) Thermo Fisher Scientific Cat#D1306 FM4-64 Thermo Fisher Scientific Cat#T13320 Ampicillin Thermo Fisher Scientific Cat#BP1760-25 Isopropylthio-b-galactoside (IPTG) Teknova Cat#I3305 Deposited data Mendeley DataSet This study https://doi.org/10.17632/skf8x9kkz8.1 Recombinant DNA pDSW206 plasmid David Weiss [44] N/A Table S1 Software and algorithms DeltaVision SoftWoRx 6.5.2 GE HealthCare incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/7.0.0/SoftWoRx.htm FIJI ver 2.1.0/1.53c ImageJ [45] imagej.net/software/fiji/ GraphPad Prism version 9.2.0 for Mac OS X GraphPad Software, San Diego, California USA. www.graphpad.com MEGA X 10.2.6 MEGA [46,47] www.megasoftware.net Microsoft Excel Microsoft Office www.microsoft.com/en-us/microsoft-365/excel Microsoft Powerpoint Microsoft Office www.microsoft.com/en-us/microsoft-365/ powerpoint Adobe Photoshop Adobe www.adobe.com/products/photoshop.html Adobe Illustrator Adobe www.adobe.com/products/illustrator.html SerialEM ver 3.8b11 [48] bio3d.colorado.edu/SerialEM/ Warp ver 1.09 [49] www.warpem.com/warp/ IMOD Package ver 4.10.28 [50] bio3d.colorado.edu/imod/ MATLAB 2019b MathWorks www.mathworks.com Dynamo ver 1.1514 [51] wiki.dynamo.biozentrum.unibas.ch dynamo2m ver 0.2.2 [52] github.com/alisterburt/dynamo2m RELION ver 3.1.1 [53,54] relion.readthedocs.io/en/release-3.1/ TomoSegMemTV [55] sites.google.com/site/3demimageprocessing/ tomosegmemtv Amira ver 6.7 Thermo Fisher Scientific www.thermofisher.com/us/en/home/electron- microscopy/products/software-em-3d-vis/ amira-software.html ChimeraX ver 1.2.1 [56] www.rbvi.ucsf.edu/chimerax/ ..
Plasmid Preparation:Article Title: A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MG1655 CGSC CGSC 6300 E. coli APEC 2248 (APEC) DSMZ DSM 103255 NEB5-alpha competent E. coli New England Biolabs Cat#C2987H vB_EcoM_Goslar Johannes Wittmann (DSMZ) [29] DSM 104658 Chemicals, peptides, and recombinant proteins DAPI (40,6-Diamidino-2Phenylindole,Dihydrochloride) Thermo Fisher Scientific Cat#D1306 FM4-64 Thermo Fisher Scientific Cat#T13320 Ampicillin Thermo Fisher Scientific Cat#BP1760-25 Isopropylthio-b-galactoside (IPTG) Teknova Cat#I3305 Deposited data Mendeley DataSet This study https://doi.org/10.17632/skf8x9kkz8.1 Recombinant DNA pDSW206 plasmid David Weiss [44] N/A Table S1 Software and algorithms DeltaVision SoftWoRx 6.5.2 GE HealthCare incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/7.0.0/SoftWoRx.htm FIJI ver 2.1.0/1.53c ImageJ [45] imagej.net/software/fiji/ GraphPad Prism version 9.2.0 for Mac OS X GraphPad Software, San Diego, California USA. www.graphpad.com MEGA X 10.2.6 MEGA [46,47] www.megasoftware.net Microsoft Excel Microsoft Office www.microsoft.com/en-us/microsoft-365/excel Microsoft Powerpoint Microsoft Office www.microsoft.com/en-us/microsoft-365/ powerpoint Adobe Photoshop Adobe www.adobe.com/products/photoshop.html Adobe Illustrator Adobe www.adobe.com/products/illustrator.html SerialEM ver 3.8b11 [48] bio3d.colorado.edu/SerialEM/ Warp ver 1.09 [49] www.warpem.com/warp/ IMOD Package ver 4.10.28 [50] bio3d.colorado.edu/imod/ MATLAB 2019b MathWorks www.mathworks.com Dynamo ver 1.1514 [51] wiki.dynamo.biozentrum.unibas.ch dynamo2m ver 0.2.2 [52] github.com/alisterburt/dynamo2m RELION ver 3.1.1 [53,54] relion.readthedocs.io/en/release-3.1/ TomoSegMemTV [55] sites.google.com/site/3demimageprocessing/ tomosegmemtv Amira ver 6.7 Thermo Fisher Scientific www.thermofisher.com/us/en/home/electron- microscopy/products/software-em-3d-vis/ amira-software.html ChimeraX ver 1.2.1 [56] www.rbvi.ucsf.edu/chimerax/ ..
Software:Article Title: A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MG1655 CGSC CGSC 6300 E. coli APEC 2248 (APEC) DSMZ DSM 103255 NEB5-alpha competent E. coli New England Biolabs Cat#C2987H vB_EcoM_Goslar Johannes Wittmann (DSMZ) [29] DSM 104658 Chemicals, peptides, and recombinant proteins DAPI (40,6-Diamidino-2Phenylindole,Dihydrochloride) Thermo Fisher Scientific Cat#D1306 FM4-64 Thermo Fisher Scientific Cat#T13320 Ampicillin Thermo Fisher Scientific Cat#BP1760-25 Isopropylthio-b-galactoside (IPTG) Teknova Cat#I3305 Deposited data Mendeley DataSet This study https://doi.org/10.17632/skf8x9kkz8.1 Recombinant DNA pDSW206 plasmid David Weiss [44] N/A Table S1 Software and algorithms DeltaVision SoftWoRx 6.5.2 GE HealthCare incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/7.0.0/SoftWoRx.htm FIJI ver 2.1.0/1.53c ImageJ [45] imagej.net/software/fiji/ GraphPad Prism version 9.2.0 for Mac OS X GraphPad Software, San Diego, California USA. www.graphpad.com MEGA X 10.2.6 MEGA [46,47] www.megasoftware.net Microsoft Excel Microsoft Office www.microsoft.com/en-us/microsoft-365/excel Microsoft Powerpoint Microsoft Office www.microsoft.com/en-us/microsoft-365/ powerpoint Adobe Photoshop Adobe www.adobe.com/products/photoshop.html Adobe Illustrator Adobe www.adobe.com/products/illustrator.html SerialEM ver 3.8b11 [48] bio3d.colorado.edu/SerialEM/ Warp ver 1.09 [49] www.warpem.com/warp/ IMOD Package ver 4.10.28 [50] bio3d.colorado.edu/imod/ MATLAB 2019b MathWorks www.mathworks.com Dynamo ver 1.1514 [51] wiki.dynamo.biozentrum.unibas.ch dynamo2m ver 0.2.2 [52] github.com/alisterburt/dynamo2m RELION ver 3.1.1 [53,54] relion.readthedocs.io/en/release-3.1/ TomoSegMemTV [55] sites.google.com/site/3demimageprocessing/ tomosegmemtv Amira ver 6.7 Thermo Fisher Scientific www.thermofisher.com/us/en/home/electron- microscopy/products/software-em-3d-vis/ amira-software.html ChimeraX ver 1.2.1 [56] www.rbvi.ucsf.edu/chimerax/ ..
Microscopy:Article Title: A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MG1655 CGSC CGSC 6300 E. coli APEC 2248 (APEC) DSMZ DSM 103255 NEB5-alpha competent E. coli New England Biolabs Cat#C2987H vB_EcoM_Goslar Johannes Wittmann (DSMZ) [29] DSM 104658 Chemicals, peptides, and recombinant proteins DAPI (40,6-Diamidino-2Phenylindole,Dihydrochloride) Thermo Fisher Scientific Cat#D1306 FM4-64 Thermo Fisher Scientific Cat#T13320 Ampicillin Thermo Fisher Scientific Cat#BP1760-25 Isopropylthio-b-galactoside (IPTG) Teknova Cat#I3305 Deposited data Mendeley DataSet This study https://doi.org/10.17632/skf8x9kkz8.1 Recombinant DNA pDSW206 plasmid David Weiss [44] N/A Table S1 Software and algorithms DeltaVision SoftWoRx 6.5.2 GE HealthCare incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/7.0.0/SoftWoRx.htm FIJI ver 2.1.0/1.53c ImageJ [45] imagej.net/software/fiji/ GraphPad Prism version 9.2.0 for Mac OS X GraphPad Software, San Diego, California USA. www.graphpad.com MEGA X 10.2.6 MEGA [46,47] www.megasoftware.net Microsoft Excel Microsoft Office www.microsoft.com/en-us/microsoft-365/excel Microsoft Powerpoint Microsoft Office www.microsoft.com/en-us/microsoft-365/ powerpoint Adobe Photoshop Adobe www.adobe.com/products/photoshop.html Adobe Illustrator Adobe www.adobe.com/products/illustrator.html SerialEM ver 3.8b11 [48] bio3d.colorado.edu/SerialEM/ Warp ver 1.09 [49] www.warpem.com/warp/ IMOD Package ver 4.10.28 [50] bio3d.colorado.edu/imod/ MATLAB 2019b MathWorks www.mathworks.com Dynamo ver 1.1514 [51] wiki.dynamo.biozentrum.unibas.ch dynamo2m ver 0.2.2 [52] github.com/alisterburt/dynamo2m RELION ver 3.1.1 [53,54] relion.readthedocs.io/en/release-3.1/ TomoSegMemTV [55] sites.google.com/site/3demimageprocessing/ tomosegmemtv Amira ver 6.7 Thermo Fisher Scientific www.thermofisher.com/us/en/home/electron- microscopy/products/software-em-3d-vis/ amira-software.html ChimeraX ver 1.2.1 [56] www.rbvi.ucsf.edu/chimerax/ ..
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